PHF21B Antibody (OTI3D1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00960
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3D1
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PHF21B(NP_612424) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PHF21B Antibody (OTI3D1)
Western Blot: PHF21B Antibody (OTI3D1) [NBP2-00960]
Western Blot: PHF21B Antibody (OTI3D1) [NBP2-00960] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHF21B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHF21B.Immunocytochemistry/ Immunofluorescence: PHF21B Antibody (OTI3D1) [NBP2-00960]
Immunocytochemistry/Immunofluorescence: PHF21B Antibody (OTI3D1) [NBP2-00960] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PHF21B .Immunohistochemistry-Paraffin: PHF21B Antibody (OTI3D1) [NBP2-00960]
Immunohistochemistry-Paraffin: PHF21B Antibody (OTI3D1) [NBP2-00960] - Staining of paraffin-embedded Human liver tissue using anti-PHF21B mouse monoclonal antibody.Applications for PHF21B Antibody (OTI3D1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PHF21B
Alternate Names
BHC80L, FLJ34161, KIAA1661, PHD finger protein 21B, PHD finger protein 4, PHF4
Entrez Gene IDs
112885 (Human)
Gene Symbol
PHF21B
Additional PHF21B Products
Product Documents for PHF21B Antibody (OTI3D1)
Product Specific Notices for PHF21B Antibody (OTI3D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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