PHF22 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92263

Novus Biologicals, part of Bio-Techne
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NBP1-92263
NBP1-92263-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHF22 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KRPADKMKSDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCH

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24058608)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF22 Antibody

Western Blot: PHF22 Antibody [NBP1-92263]

Western Blot: PHF22 Antibody [NBP1-92263]

Western Blot: PHF22 Antibody [NBP1-92263] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: PHF22 Antibody [NBP1-92263]

Immunocytochemistry/ Immunofluorescence: PHF22 Antibody [NBP1-92263]

Immunocytochemistry/Immunofluorescence: PHF22 Antibody [NBP1-92263] - Staining of human cell line A-431 shows localization to nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PHF22 Antibody [NBP1-92263]

Immunohistochemistry-Paraffin: PHF22 Antibody [NBP1-92263]

Immunohistochemistry-Paraffin: PHF22 Antibody [NBP1-92263] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for PHF22 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF22

INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM]

Alternate Names

Int12, integrator complex subunit 12, PHD finger protein 22INT12, PHF22, SBBI22

Gene Symbol

INTS12

Additional PHF22 Products

Product Documents for PHF22 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHF22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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