PHF7 Antibody (OTI1G9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73371

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PHF7 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7(NP_057567) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHF7 Antibody (OTI1G9) - Azide and BSA Free

Western Blot: PHF7 Antibody (OTI1G9)Azide and BSA Free [NBP2-73371]

Western Blot: PHF7 Antibody (OTI1G9)Azide and BSA Free [NBP2-73371]

Western Blot: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHF7.
Immunocytochemistry/ Immunofluorescence: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371]

Immunocytochemistry/ Immunofluorescence: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371]

Immunocytochemistry/Immunofluorescence: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PHF7.
Flow Cytometry: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371]

Flow Cytometry: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371]

Flow Cytometry: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by PHF7 antibody.
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Applications for PHF7 Antibody (OTI1G9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PHF7

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Alternate Names

DKFZp434L1850, HSPC045, HSPC226, MGC26088, NYD-SP6, PHD finger protein 7, Testis development protein NYD-SP6

Gene Symbol

PHF7

Additional PHF7 Products

Product Documents for PHF7 Antibody (OTI1G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PHF7 Antibody (OTI1G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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