PHF8 Antibody (2H9D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15910

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15910-100ul
NBP3-15910-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2H9D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PHF8 Antibodies »

Product Summary for PHF8 Antibody (2H9D4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human PHF8 (Q9UPP1). TVSNSPASQRTPGKRPIKRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF8 Antibody (2H9D4)

Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910]

Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910]

Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910] - Western blot analysis of extracts of HeLa cells, using PHF8 antibody (NBP3-15910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910]

Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910]

Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910] - Western blot analysis of extracts of various cell lines, using PHF8 antibody (NBP3-15910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for PHF8 Antibody (2H9D4)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PHF8

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Long Name

Histone lysine demethylase PHF8

Alternate Names

KIAA1111, ZNF422

Gene Symbol

PHF8

Additional PHF8 Products

Product Documents for PHF8 Antibody (2H9D4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PHF8 Antibody (2H9D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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