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PHF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93314

Novus Biologicals, part of Bio-Techne
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NB100-93314

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 925 and 975 of human PHD finger protein 8 using the numbering given in entry CAI41576.1 (GeneID 23133).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF8 Antibody

Western Blot: PHF8 Antibody [NB100-93314]

Western Blot: PHF8 Antibody [NB100-93314]

Western Blot: PHF8 Antibody [NB100-93314] - Detection of Human PHF8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (RIPA), 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PHF8 antibody NB100-93314 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Knockdown Validated: PHF8 Antibody [NB100-93314]

Knockdown Validated: PHF8 Antibody [NB100-93314]

Knockdown Validated: PHF8 Antibody [NB100-93314] - Western blot analysis showing knockdown of PHF8 by shRNA against PHF8. LNCaP cells were infected with a lentiviral shPHF8 and western blot analysis was carried out with anti-PHF8 antibody. Image collected and cropped by CiteAb from the llowing publication (nature.com/articles/oncsis201674), licensed under a CC-BY license.
PHF8 Antibody

Western Blot: PHF8 Antibody [NB100-93314] -

Western Blot: PHF8 Antibody [NB100-93314] - PHF8 interacts with the AR & functions as an AR coactivator. (a) PHF8 interacts with AR independent of DHT. HEK293T cells were transiently transfected with plasmids expressing Flag-tagged PHF8 & GFP-tagged AR. Cells were treated with or without 10 nM DHT for 48 h & the cell lysates were used for immunoprecipitation with antibodies against either Flag or GFP. Western blotting was conducted with antibodies as indicated. (b) PHF8 interacts with AR independent of its demethylase activity. HEK293T cells were transiently transfected with plasmids expressing Flag-tagged PHF8 (H247A) mutant & GFP-tagged AR. The interaction assay by co-immunoprecipitation was performed as described above. (c) In LNCaP cells endogenous PHF8 interacts with AR. LNCaP cells were treated with 10 nM DHT for 48 h & the cell lysates were used for immunoprecipitation with antibodies against either PHF8 or AR. Western blotting was conducted with AR or PHF8 antibody as indicated. (d) PHF8 enhances AR transcriptional activation in a demethylase activity-dependent manner. HeLa cells were co-transfected with the 4 × UAS-TK-luc reporter, the plasmids expressing the fusion protein Gal-AR, & increased amount of either the wild-type PHF8 or mutant PHF8 (H247A) as indicated. Cells were cultured with or without 10 nM R1881 overnight. The relative luciferase activities in the cell lysates were normalized to renilla luciferase activity. The relative luciferase activities are presented as mean±s.e.m. of three independent transfections & the levels of PHF8 were estimated by western blot as depicted in the bottom panel. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/oncsis201674), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PHF8 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 27991916). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHF8

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111

Long Name

Histone lysine demethylase PHF8

Alternate Names

KIAA1111, ZNF422

Entrez Gene IDs

23133 (Human)

Gene Symbol

PHF8

UniProt

Additional PHF8 Products

Product Documents for PHF8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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