PHGDH Antibody (CL0555)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52939
![Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-CL0555-Western-Blot-NBP2-52939-img0007.jpg "Western Blot: PHGDH Antibody (CL0555) [NBP2-52939]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0555
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDM
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PHGDH Antibody (CL0555)
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] -
Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4![Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-CL0555-Immunocytochemistry-Immunofluorescence-NBP2-52939-img0009.jpg "Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939]")
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939]
Immunocytochemistry/Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939] - Staining of MCF7 cells using the Anti-PHGDH monoclonal antibody, showing specific staining in the cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Applications for PHGDH Antibody (CL0555)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Gene Symbol
PHGDH
Additional PHGDH Products
Product Documents for PHGDH Antibody (CL0555)
Product Specific Notices for PHGDH Antibody (CL0555)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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