PHGDH Antibody

Western Blot: PHGDH Antibody [NBP1-87311]

Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skeletal muscle shows no positivity as expected.

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.

Simple Western: PHGDH Antibody [NBP1-87311]

Simple Western: PHGDH Antibody [NBP1-87311] - Simple Western lane view shows a specific band for PHGDH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: PHGDH Antibody [NBP1-87311]

Simple Western: PHGDH Antibody [NBP1-87311] - Electropherogram image(s) of corresponding Simple Western lane view. PHGDH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Western Blot: PHGDH Antibody [NBP1-87311] -

Knockdown of PNKP or TDP1 leads to increase in expression of PSAT1 and PHGDH. Western blot analysis of XRCC1, PHGDH and PSAT1 expression upon KD of PNKP (A) or TDP1 (B). beta-Actin served as a loading control. (C) TDP1 mRNA levels after KD of TDP1, quantified by qPCR and normalised to GAPDH.

Western Blot: PHGDH Antibody [NBP1-87311] -

Western Blot: PHGDH Antibody [NBP1-87311] - Knockdown of PNKP or TDP1 leads to increase in expression of PSAT1 & PHGDH. Western blot analysis of XRCC1, PHGDH & PSAT1 expression upon KD of PNKP (A) or TDP1 (B). beta-Actin served as a loading control. (C) TDP1 mRNA levels after KD of TDP1, quantified by qPCR & normalised to GAPDH. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25800737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.