PHGDH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19801

Novus Biologicals, part of Bio-Techne
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NBP2-19801
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Mouse (96%), Porcine (90%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHGDH Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Bovine (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHGDH Antibody

Knockout Validated: PHGDH Antibody [NBP2-19801]

Knockout Validated: PHGDH Antibody [NBP2-19801]

Knockout Validated: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: PHGDH Antibody [NBP2-19801]

Western Blot: PHGDH Antibody [NBP2-19801]

Western Blot: PHGDH Antibody [NBP2-19801] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801]

Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801]

Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801] - Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody [N3C2], Internal diluted at 1:250. Blue: Fluoroshield with DAPI.
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Applications for PHGDH Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHGDH

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Long Name

3-Phosphoglycerate Dehydrogenase

Alternate Names

3PGDH, PDGH3

Gene Symbol

PHGDH

UniProt

O43175 (Human)

Additional PHGDH Products

Product Documents for PHGDH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHGDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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