PHGDH Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18850
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PHGDH Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human PHGDH fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
Scientific Data Images for PHGDH Antibody
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] -
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human skin with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP3-18850] -
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP3-18850] - Immunofluorescence staining of PHGDH in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-18850 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] -
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human breast carcinoma with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.Applications for PHGDH Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS, pH 7.0
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Gene Symbol
PHGDH
Additional PHGDH Products
Product Specific Notices for PHGDH Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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