Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PHLDB2 Antibodies »

Product Specifications

Immunogen

The immungoen recognized by the antibody maps to a region between residue 250 to 300 of human LL5beta using the numbering given in entry NP_001127910.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHLDB2 Antibody

Western Blot: PHLDB2 Antibody [NBP2-36546]

Western Blot: PHLDB2 Antibody [NBP2-36546]

Western Blot: PHLDB2 Antibody [NBP2-36546] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LL5beta antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes
Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]

Immunoprecipitation: PHLDB2 Antibody [NBP2-36546]

Immunoprecipitation: PHLDB2 Antibody [NBP2-36546] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated LL5beta, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PHLDB2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHLDB2

Pleckstrin homology-like domain family B member 2 (LL5beata) seems to be involved in the assembly of the postsynaptic apparatus. LL5beta may play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane (By similarity).

Alternate Names

pleckstrin homology-like domain, family B, member 2

Gene Symbol

PHLDB2

UniProt

NP_001127910.1 (Human)

Additional PHLDB2 Products

Product Documents for PHLDB2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHLDB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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