PHLDB2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38238
![Western Blot: PHLDB2 Antibody [NBP2-38238]](https://resources.bio-techne.com/images/products/PHLDB2-Antibody-Western-Blot-NBP2-38238-img0009.jpg "Western Blot: PHLDB2 Antibody [NBP2-38238]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot
Cited:
Immunohistochemistry-Paraffin, Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATM
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHLDB2 Antibody
![Immunocytochemistry/ Immunofluorescence: PHLDB2 Antibody [NBP2-38238]](https://resources.bio-techne.com/images/products/PHLDB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38238-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PHLDB2 Antibody [NBP2-38238]")
Immunocytochemistry/ Immunofluorescence: PHLDB2 Antibody [NBP2-38238]
Immunocytochemistry/Immunofluorescence: PHLDB2 Antibody [NBP2-38238] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: PHLDB2 Antibody [NBP2-38238]](https://resources.bio-techne.com/images/products/PHLDB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38238-img0007.jpg "Immunohistochemistry-Paraffin: PHLDB2 Antibody [NBP2-38238]")
Immunohistochemistry-Paraffin: PHLDB2 Antibody [NBP2-38238]
Immunohistochemistry-Paraffin: PHLDB2 Antibody [NBP2-38238] - Staining of human stomach, upper shows strong cytoplasmic positivity in granular pattern in glandular cells.Applications for PHLDB2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
Use in Proximity Ligation Assay reported in scientific literature (PMID:34952201).For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHLDB2
Alternate Names
pleckstrin homology-like domain, family B, member 2
Gene Symbol
PHLDB2
UniProt
Additional PHLDB2 Products
Product Documents for PHLDB2 Antibody
Product Specific Notices for PHLDB2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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