Phospho-Axl (Y779) Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6965G
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 713610
Product Specifications
Immunogen
Phosphopeptide containing the human Axl Y779 site
Specificity
Detects human Phospho-Axl (Y779) in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Phospho-Axl (Y779) Alexa Fluor® 488-conjugated Antibody
Detection of Phospho-Axl (Y779) in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line (A) resting or (B) treated with 100 nM Pervanadate for 10 minutes was stained with Mouse Anti-Human Phospho-Axl (Y779) Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6965G) and Mouse Anti-Human Axl APC-conjugated Monoclonal Antibody (Catalog # FAB154A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Phospho-Axl (Y779) Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
5 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line treated with Pervanadate was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Sample: HeLa human cervical epithelial carcinoma cell line treated with Pervanadate was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Phosphorylation of Tyrosine 779 provides a docking site for p85 subunits of PI 3-Kinase (4).
References
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
- Weinger, J.G. et al. (2008) J. Neurochem. 106:134.
Long Name
Axl Receptor Tyrosine Kinase
Alternate Names
AI323647, ARK, JTK11, Tyro7, UFO
Gene Symbol
AXL
Additional Axl Products
Product Specific Notices for Phospho-Axl (Y779) Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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