Phospho-STAT2 (Y690) Alexa Fluor® 488‑conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC8627G
Key Product Details
Validated by
Species Reactivity
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Product Specifications
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Scientific Data Images for Phospho-STAT2 (Y690) Alexa Fluor® 488‑conjugated Antibody
Detection of Phospho-STAT2 (Y690) in Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cells were serum starved ovenight, then left untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes was stained with Rabbit Anti-Human STAT2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8627G, filled histogram) or isotype control antibody (IC105G, not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.Applications for Phospho-STAT2 (Y690) Alexa Fluor® 488‑conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol
Formulation, Preparation, and Storage
Purification
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Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT2
STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha- and beta-) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.
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Additional STAT2 Products
Product Specific Notices for Phospho-STAT2 (Y690) Alexa Fluor® 488‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only