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Key Product Details

Species Reactivity

Non-species specific

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PY20

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Phosphotyrosine conjugated to KLH

Localization

Depends upon the location of phosphorylated target

Specificity

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29402).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free

Immunohistochemistry-Paraffin: Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free [NBP2-33166]

Immunohistochemistry-Paraffin: Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free [NBP2-33166]

Immunohistochemistry-Paraffin: Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free [NBP2-33166] - Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.

Applications for Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Phospho-Tyrosine

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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