PHOSPHO2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81168
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QIVYIGDGGNDVCPVTFLKNDDVAMPRKGYTLQKTLSRMSQNLEPMEYSVVVWSSGVDIISHLQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHOSPHO2 Antibody
![Western Blot: PHOSPHO2 Antibody [NBP1-81168]](https://resources.bio-techne.com/images/products/PHOSPHO2-Antibody-Western-Blot-NBP1-81168-img0005.jpg "Western Blot: PHOSPHO2 Antibody [NBP1-81168]")
Western Blot: PHOSPHO2 Antibody [NBP1-81168]
Western Blot: PHOSPHO2 Antibody [NBP1-81168] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp![Immunocytochemistry/ Immunofluorescence: PHOSPHO2 Antibody [NBP1-81168]](https://resources.bio-techne.com/images/products/PHOSPHO2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81168-img0007.jpg "Immunocytochemistry/ Immunofluorescence: PHOSPHO2 Antibody [NBP1-81168]")
Immunocytochemistry/ Immunofluorescence: PHOSPHO2 Antibody [NBP1-81168]
Immunocytochemistry/Immunofluorescence: PHOSPHO2 Antibody [NBP1-81168] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: PHOSPHO2 Antibody [NBP1-81168]](https://resources.bio-techne.com/images/products/PHOSPHO2-Antibody-Immunohistochemistry-Paraffin-NBP1-81168-img0002.jpg "Immunohistochemistry-Paraffin: PHOSPHO2 Antibody [NBP1-81168]")
Immunohistochemistry-Paraffin: PHOSPHO2 Antibody [NBP1-81168]
Immunohistochemistry-Paraffin: PHOSPHO2 Antibody [NBP1-81168] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.Applications for PHOSPHO2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHOSPHO2
Additional PHOSPHO2 Products
Product Documents for PHOSPHO2 Antibody
Product Specific Notices for PHOSPHO2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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