Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73376

Novus Biologicals, part of Bio-Techne
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Availability
Size / Price
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NBP2-73376
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Conjugate
Catalog #
Unconjugated
NBP2-02559
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Alexa Fluor 350
NBP2-73376AF350
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Alexa Fluor 405
NBP2-73376AF405
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Alexa Fluor 488
NBP2-73376AF488
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Alexa Fluor 532
NBP2-73376AF532
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Alexa Fluor 594
NBP2-73376AF594
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Alexa Fluor 647
NBP2-73376AF647
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Alexa Fluor 700
NBP2-73376AF700
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Alexa Fluor 750
NBP2-73376AF750
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Allophycocyanin
NBP2-73376APC
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Allophycocyanin/Cy7
NBP2-73376APCCY7
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Biotin
NBP2-73376B
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CoraFluor 1
NBP2-73376CL1
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DyLight 350
NBP2-73376UV
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DyLight 405
NBP2-73376V
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DyLight 488
NBP2-73376G
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DyLight 550
NBP2-73376R
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DyLight 594
NBP2-73376DL594
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DyLight 650
NBP2-73376C
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DyLight 680
NBP2-73376FR
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DyLight 755
NBP2-73376IR
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FITC
NBP2-73376F
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HRP
NBP2-73376H
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Janelia Fluor 525
NBP2-73376JF525
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Janelia Fluor 549
NBP2-73376JF549
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Janelia Fluor 585
NBP2-73376JF585
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Janelia Fluor 635
NBP2-73376JF635
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Janelia Fluor 646
NBP2-73376JF646
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Janelia Fluor 669
NBP2-73376JF669
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mFluor Violet 450 SE
NBP2-73376MFV450
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mFluor Violet 500 SE
NBP2-73376MFV500
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mFluor Violet 610 SE
NBP2-73376MFV610
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PE
NBP2-73376PE
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PE/Atto594
NBP2-73376PEATT594
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PE/Cy5.5
NBP2-73376PECY55
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PE/Cy7
NBP2-73376PECY7
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PerCP
NBP2-73376PCP
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Key Product Details

Species Reactivity

Human, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Phosphodiesterase 4A/PDE4A Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free

Western Blot: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)Azide and BSA Free [NBP2-73376]

Western Blot: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)Azide and BSA Free [NBP2-73376]

Western Blot: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.
Immunocytochemistry/ Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376]

Immunocytochemistry/ Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376]

Immunocytochemistry/Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376] - Staining of HT29 cells using anti-PDE4A mouse monoclonal antibody.
Immunohistochemistry: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376]

Immunohistochemistry: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376]

Immunohistochemistry: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free [NBP2-73376] - Staining of paraffin-embedded Human pancreas tissue using anti-PDE4A mouse monoclonal antibody.
View 8 more images

Applications for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphodiesterase 4A/PDE4A

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling.

Alternate Names

DPDE2, PDE46, PDE4A

Gene Symbol

PDE4A

Additional Phosphodiesterase 4A/PDE4A Products

Product Documents for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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