Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-635

Novus Biologicals, part of Bio-Techne
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NB300-635-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Mouse

Applications

Validated:

ELISA, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Phosphodiesterase 4A/PDE4A Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine.

Modification

p Ser686, p Ser688

Localization

Cell Membrane

Specificity

This detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with PDE4B, PDE4D or any other member of the PDE family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635]

Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635]

Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635] - Lane 1: PDE4A Western blot positive Control (PDE4A5); Lane 2: Western blot with Phospho-PDE4A5

Applications for Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

Application
Recommended Usage

ELISA

1:10000

Immunoprecipitation

1:150

Western Blot

1:500
Application Notes
Immunoprecipitation and Western Blot: Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions and concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphodiesterase 4A/PDE4A

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling.

Alternate Names

DPDE2, PDE46, PDE4A

Entrez Gene IDs

5141 (Human); 18577 (Mouse); 25638 (Rat)

Gene Symbol

PDE4A

UniProt

P27815 (Human)

Additional Phosphodiesterase 4A/PDE4A Products

Product Documents for Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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