Phospholamban Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85543

Novus Biologicals, part of Bio-Techne
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NBP1-85543
NBP1-85543-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Phospholamban Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKVQYLTRSAIRRASTIEMPQQARQKLQNL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospholamban Antibody

Western Blot: Phospholamban Antibody [NBP1-85543]

Western Blot: Phospholamban Antibody [NBP1-85543]

Western Blot: Phospholamban Antibody [NBP1-85543] - Analysis in human heart tissue.
Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543]

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543]

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543]

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543]

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543] - Staining of human heart muscle shows high expression.
View 1 more images

Applications for Phospholamban Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholamban

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Gene Symbol

PLN

Additional Phospholamban Products

Product Documents for Phospholamban Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospholamban Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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