Phospholamban [p Ser16] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18744
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine
Modification
p Ser16
Specificity
Recognizes phosphorylated phospholamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20C may cause glycerol containing solutions to become frozen during storage. Stable for 1 year at -20C from date of receipt.
Scientific Data Images for Phospholamban [p Ser16] Antibody
![Phospholamban [p Ser16] Antibody](https://resources.bio-techne.com/images/products/antibody/nbp3-18744_rabbit-phospholamban-p-ser16-pab-western-blot-1992023132527..jpg "Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-")
Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-
Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-Bovine cardiac preparation treated with PKA (lane1) or untreated (lane 2), was probed with anti-phospho-phospholamban (0.5 ug/ml).Applications for Phospholamban [p Ser16] Antibody
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Western Blot: Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA
0.5-2 ug/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 ug PKA for 10 minutes. DO NOT BOIL CARDIAC PREPARATION. Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
0.5-2 ug/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 ug PKA for 10 minutes. DO NOT BOIL CARDIAC PREPARATION. Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, and 30% glycerol.
Preservative
0.07% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Phospholamban
Additional Phospholamban Products
Product Documents for Phospholamban [p Ser16] Antibody
Product Specific Notices for Phospholamban [p Ser16] Antibody
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20C may cause glycerol containing solutions to become frozen during storage. Stable for 1 year at -20C from date of receipt.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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