Phospholipase D2 Antibody (1C5)
Novus Biologicals, part of Bio-Techne | Catalog # H00005338-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1C5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PLD2 (AAH15033, 834 a.a. ~ 933 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT
Specificity
PLD2 - phospholipase D2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Phospholipase D2 Antibody (1C5)
![Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]](https://resources.bio-techne.com/images/products/Phospholipase-D2-Antibody-1C5-Western-Blot-H00005338-M01-img0005.jpg "Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]")
Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]
Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01] - Analysis of PLD2 expression in transfected 293T cell line by PLD2 monoclonal antibody (M01), clone 1C5.Lane 1: PLD2 transfected lysate (Predicted MW: 105.9 KDa).Lane 2: Non-transfected lysate.![Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]](https://resources.bio-techne.com/images/products/Phospholipase-D2-Antibody-1C5-Western-Blot-H00005338-M01-img0004.jpg "Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]")
Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]
Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01] - PLD2 monoclonal antibody (M01), clone 1C5. Analysis of PLD2 expression in rat brain.![ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01]](https://resources.bio-techne.com/images/products/Phospholipase-D2-Antibody-1C5-ELISA-H00005338-M01-img0006.jpg "ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01]")
ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01]
ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01] - Detection limit for recombinant GST tagged PLD2 is approximately 0.03ng/ml as a capture antibody.Applications for Phospholipase D2 Antibody (1C5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Phospholipase D2
Alternate Names
Choline phosphatase 2, EC 3.1.4.4, hPLD2, Phosphatidylcholine-hydrolyzing phospholipase D2, phospholipase D2, PLD 2, PLD1C
Entrez Gene IDs
5338 (Human)
Gene Symbol
PLD2
OMIM
602384 (Human)
UniProt
Additional Phospholipase D2 Products
Product Documents for Phospholipase D2 Antibody (1C5)
Product Specific Notices for Phospholipase D2 Antibody (1C5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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