Phospholipase D2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23721
Key Product Details
Species Reactivity
Validated:
Human, Primate
Predicted:
Chimpanzee (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Summary for Phospholipase D2 Antibody
Immunogen
Within the range amino acids 199-230 of human PLD2 protein were used as the immunogen.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: rhesus maque (81%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Phospholipase D2 Antibody
![Immunohistochemistry-Paraffin: Phospholipase D2 Antibody [NBP2-23721]](https://resources.bio-techne.com/images/products/Phospholipase-D2-Antibody-Immunohistochemistry-Paraffin-NBP2-23721-img0001.jpg "Immunohistochemistry-Paraffin: Phospholipase D2 Antibody [NBP2-23721]")
Immunohistochemistry-Paraffin: Phospholipase D2 Antibody [NBP2-23721]
Immunohistochemistry-Paraffin: Phospholipase D2 Antibody [NBP2-23721] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using Phospholipase D2 antibody at 1:100 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.Applications for Phospholipase D2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:100
Immunohistochemistry-Paraffin
1:50 - 1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
0.1 ml crude whole Rabbit sera with 50% Glycerol
Preservative
0.025% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Phospholipase D2
Alternate Names
Choline phosphatase 2, EC 3.1.4.4, hPLD2, Phosphatidylcholine-hydrolyzing phospholipase D2, phospholipase D2, PLD 2, PLD1C
Gene Symbol
PLD2
Additional Phospholipase D2 Products
Product Documents for Phospholipase D2 Antibody
Product Specific Notices for Phospholipase D2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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