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Phosphopantothenate-cysteine ligase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87398

Novus Biologicals, part of Bio-Techne
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NBP1-87398
NBP1-87398-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLADY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphopantothenate-cysteine ligase Antibody

Western Blot: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398]

Western Blot: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398]

Western Blot: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398] - Analysis using Anti-PPCS antibody NBP1-87398 (A) shows similar pattern to independent antibody NBP2-38181 (B).
Immunocytochemistry/ Immunofluorescence: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398]

Immunocytochemistry/ Immunofluorescence: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398]

Immunocytochemistry/Immunofluorescence: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398] - Staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398]

Immunohistochemistry-Paraffin: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398]

Immunohistochemistry-Paraffin: Phosphopantothenate-cysteine ligase Antibody [NBP1-87398] - Staining of human smooth muscle shows distinct cytoplasmic positivity in smooth muscle cells.

Applications for Phosphopantothenate-cysteine ligase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphopantothenate-cysteine ligase

Alternate Names

FLJ11838, MGC117357, phosphopantothenoylcysteine synthetase, PPC synthetase

Gene Symbol

PPCS

Additional Phosphopantothenate-cysteine ligase Products

Product Documents for Phosphopantothenate-cysteine ligase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phosphopantothenate-cysteine ligase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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