Phosphorylase B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57900

Novus Biologicals, part of Bio-Techne
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NBP2-57900
NBP2-57900-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Phosphorylase B Antibodies »

Product Summary for Phosphorylase B Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: AALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFLRDGYRT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphorylase B Antibody

Immunocytochemistry/ Immunofluorescence: Phosphorylase B Antibody [NBP2-57900]

Immunocytochemistry/ Immunofluorescence: Phosphorylase B Antibody [NBP2-57900]

Immunocytochemistry/Immunofluorescence: Phosphorylase B Antibody [NBP2-57900] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus.

Applications for Phosphorylase B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphorylase B

Phosphorylase B, also known by its gene name, PHKB, is an enzyme localized to the plasma membrane that catalyzes the phosphorylation of the amino acid residue Serine. Phosphorylase B has a 1,093 amino acid long isoform that is 125 kDa (the canonical sequence) and three other isoforms derived from alternative splicing. Current research on PHKB is being performed relating to several diseases and disorders including hepatitis and metabolic disorders, such as hypoglycemia and glycogen storage disease. Phosphorylase B has also been shown to have interactions with B Raf, hnRNP C1 + C2, PASK, and UBE3A in pathways such as Glucose and Glycogen Metabolism, PKA Signaling, cAMP Pathway, Insulin Signaling Pathway and Calcium Signaling Pathway.

Alternate Names

DKFZp781E15103, FLJ41698, phosphorylase b kinase regulatory subunit beta, phosphorylase kinase beta-subunit, Phosphorylase kinase subunit beta, phosphorylase kinase, beta

Gene Symbol

PHKB

Additional Phosphorylase B Products

Product Documents for Phosphorylase B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphorylase B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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