Phosphorylase B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93725

Novus Biologicals, part of Bio-Techne
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NBP2-93725-0.02ml
NBP2-93725-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Phosphorylase B Antibodies »

Product Summary for Phosphorylase B Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 854-1093 of human PHKB (NP_000284.1). VIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQLLLDILQPQQNGRCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphorylase B Antibody - BSA Free

Western Blot: Phosphorylase B AntibodyBSA Free [NBP2-93725]

Western Blot: Phosphorylase B AntibodyBSA Free [NBP2-93725]

Western Blot: Phosphorylase B Antibody [NBP2-93725] - Analysis of extracts of various cell lines, using Phosphorylase B at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Applications for Phosphorylase B Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphorylase B

Phosphorylase B, also known by its gene name, PHKB, is an enzyme localized to the plasma membrane that catalyzes the phosphorylation of the amino acid residue Serine. Phosphorylase B has a 1,093 amino acid long isoform that is 125 kDa (the canonical sequence) and three other isoforms derived from alternative splicing. Current research on PHKB is being performed relating to several diseases and disorders including hepatitis and metabolic disorders, such as hypoglycemia and glycogen storage disease. Phosphorylase B has also been shown to have interactions with B Raf, hnRNP C1 + C2, PASK, and UBE3A in pathways such as Glucose and Glycogen Metabolism, PKA Signaling, cAMP Pathway, Insulin Signaling Pathway and Calcium Signaling Pathway.

Alternate Names

DKFZp781E15103, FLJ41698, phosphorylase b kinase regulatory subunit beta, phosphorylase kinase beta-subunit, Phosphorylase kinase subunit beta, phosphorylase kinase, beta

Gene Symbol

PHKB

Additional Phosphorylase B Products

Product Documents for Phosphorylase B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphorylase B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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