Phosphoserine Antibody (PSR-45)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-405

Novus Biologicals, part of Bio-Techne
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NB600-405
NB600-405-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Rabbit

Cited:

Rabbit

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PSR-45

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Phosphoserine Antibodies »

Product Specifications

Immunogen

Phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 26003536)

Specificity

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phosphoserine Antibody (PSR-45)

Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405]

Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405]

Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)

Applications for Phosphoserine Antibody (PSR-45)

Application
Recommended Usage

ELISA

1:4000

Western Blot

1:500-1:1000
Application Notes
Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. The ELISA dilution given is for indirect.

Reviewed Applications

Read 1 review rated 5 using NB600-405 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphoserine

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Alternate Names

Phosphoserine; Phosphothreonine

Additional Phosphoserine Products

Product Documents for Phosphoserine Antibody (PSR-45)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphoserine Antibody (PSR-45)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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