Phosphothreonine Antibody (RM102) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25980

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25980
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Key Product Details

Validated by

Biological Validation

Species Reactivity

All Species

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM102

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Phosphothreonine Antibodies »

Product Specifications

Immunogen

Mixture of Phosphothreonine-BSA conjugate and a Phosphothreonine containing peptide

Specificity

This antibody reacts threonine-phosphorylated proteins. No cross reactivity with non-phosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphothreonine Antibody (RM102) - Azide and BSA Free

Phosphothreonine Antibody (RM102) - Azide and BSA Free

Immunoprecipitation: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] -

Immunoprecipitation: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by clone RM102 rabbit Anti-Phosphothreonine mAb, at 1:500 dilution, and then blotted with RM102. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.
Phosphothreonine Antibody (RM102) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] -

Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1:500 dilution followed by a PE conjugated secondary antibody (red) and DAPI (blue).
Phosphothreonine Antibody (RM102) - Azide and BSA Free

Western Blot: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] -

Western Blot: Phosphothreonine Antibody (RM102) - Azide and BSA Free [NBP3-25980] - Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1:2000 dilution.
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Applications for Phosphothreonine Antibody (RM102) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100-1:500

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

1:100-1:500

Western Blot

1:500 -1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphothreonine

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.

Alternate Names

C4H10NO6P;O-phospho-L-threonine;Phospho-threonine;pThr;Threonine

Additional Phosphothreonine Products

Product Documents for Phosphothreonine Antibody (RM102) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphothreonine Antibody (RM102) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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