Phosphothreonine Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96896

Novus Biologicals, part of Bio-Techne
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NB110-96896
NB110-96896-100ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.25 mg/ml

View all Phosphothreonine Antibodies »

Product Specifications

Immunogen

Phosphothreonine conjugated to KLH

Specificity

Recognizes proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphothreonine Antibody

Western Blot: Phosphothreonine Antibody [NB110-96896]

Western Blot: Phosphothreonine Antibody [NB110-96896]

Western Blot: Phosphothreonine Antibody [NB110-96896] - Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896) at 1:1000. Left: Treated with Vanadium, Right: Non-treated..
Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody [NB110-96896]

Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody [NB110-96896]

Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x.
Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody [NB110-96896]

Immunocytochemistry/ Immunofluorescence: Phosphothreonine Antibody [NB110-96896]

Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.

Applications for Phosphothreonine Antibody

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:60

Immunoprecipitation

1:100

Western Blot

1:500

Reviewed Applications

Read 2 reviews rated 4 using NB110-96896 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% Glycerol

Preservative

0.01% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phosphothreonine

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).

Alternate Names

C4H10NO6P;O-phospho-L-threonine;Phospho-threonine;pThr;Threonine

Additional Phosphothreonine Products

Product Documents for Phosphothreonine Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphothreonine Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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