PHOX2A Antibody (4F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00000401-M01

Novus Biologicals, part of Bio-Techne
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H00000401-M01
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockdown Validated, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHOX2A Antibodies »

Product Specifications

Immunogen

PHOX2A (NP_005160, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDYSYLNSYDSCVAAMEASAYGDFGACSQPGGFQYSPLRPAFPAAGPPCPALGSSNCALGALRDHQPAPYSAVPYKFFPEPSGLHEKRKQ

Specificity

PHOX2A - paired-like (aristaless) homeobox 2a

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PHOX2A Antibody (4F6)

Western Blot: PHOX2A Antibody (4F6) [H00000401-M01]

Western Blot: PHOX2A Antibody (4F6) [H00000401-M01]

Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A expression in transfected 293T cell line by PHOX2A monoclonal antibody (M01), clone 4F6.Lane 1: PHOX2A transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PHOX2A Antibody (4F6) [H00000401-M01]

Immunocytochemistry/ Immunofluorescence: PHOX2A Antibody (4F6) [H00000401-M01]

Immunocytochemistry/Immunofluorescence: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of monoclonal antibody to PHOX2A on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PHOX2A Antibody (4F6) [H00000401-M01]

Western Blot: PHOX2A Antibody (4F6) [H00000401-M01]

Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A over-expressed 293 cell line, cotransfected with PHOX2A Validated Chimera RNAi ( Cat # H00000401-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PHOX2A monoclonal antibody (M01), clone 4F6 (Cat # H00000401-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for PHOX2A Antibody (4F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 30315878).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHOX2A

The protein encoded by this gene contains a paired-like homeodomain most similar to that of the Drosophila aristaless gene product. The encoded protein plays a central role in development of the autonomic nervous system. It regulates the expression of tyrosine hydroxylase and dopamine beta-hydroxylase, two catecholaminergic biosynthetic enzymes essential for the differentiation and maintenance of the noradrenergic neurotransmitter phenotype. The encoded protein has also been shown to regulate transcription of the alpha3 nicotinic acetylcholine receptor gene. Mutations in this gene have been associated with autosomal recessive congenital fibrosis of the extraocular muscles.

Alternate Names

aristaless (Drosophila) homeobox, aristaless homeobox (Drosophila), fibrosis ofextraocular muscles, congenital, 2, autosomal recessive, aristaless homeobox homolog, Aristaless homeobox protein homolog, arix homeodomain protein, ARIX1 homeodomain protein, ARIXNCAM2, CFEOM2MGC52227, paired mesoderm homeobox protein 2A, paired-like homeobox 2apaired-like (aristaless) homeobox 2a, PMX2AFEOM2

Entrez Gene IDs

401 (Human)

Gene Symbol

PHOX2A

OMIM

602078 (Human)

UniProt

NP_005160 (Human)

Additional PHOX2A Products

Product Documents for PHOX2A Antibody (4F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHOX2A Antibody (4F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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