PHPT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99999

Novus Biologicals, part of Bio-Techne
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NBP2-99999-100ul
NBP2-99999-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PHPT1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human PHPT1 (Uniprot#: Q9NRX4-1; Ala2-Tyr125)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PHPT1 Antibody

Western Blot: PHPT1 Antibody [NBP2-99999]

Western Blot: PHPT1 Antibody [NBP2-99999]

Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa
Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-99999]

Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-99999]

Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-99999] - Staining of human PHPT1 in human skeletal muscle with rabbit polyclonal antibody (1:1000).
Immunoprecipitation: PHPT1 Antibody [NBP2-99999]

Immunoprecipitation: PHPT1 Antibody [NBP2-99999]

Immunoprecipitation: PHPT1 Antibody [NBP2-99999] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PHPT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PHPT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa

Applications for PHPT1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHPT1

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002 (PubMed 12383260)).(supplied by OMIM)

Alternate Names

bA216L13.10, DKFZp564M173, EC 3.1.3,14 kDa phosphohistidine phosphatase, EC 3.1.3.-, HSPC141, phosphohistidine phosphatase 11700008C22Rik, phosphohistidine phosphatase 14kDa, PHP14CGI-202, Protein janus-A homolog, RP11-216L13.10, sex-regulated protein janus-a

Gene Symbol

PHPT1

Additional PHPT1 Products

Product Documents for PHPT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHPT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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