PHYH Antibody (1F2-5B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005264-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F2-5B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHYH Antibodies »

Product Specifications

Immunogen

PHYH (AAH29512, 1 a.a. ~ 338 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQLRAAARLQIVLGHLGRPSAGAVVAHPTSGTISSASFHPQQFQYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL

Localization

peroxisome

Specificity

PHYH - phytanoyl-CoA hydroxylase (Refsum disease)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PHYH Antibody (1F2-5B9)

Western Blot: PHYH Antibody (1F2-5B9) [H00005264-M01]

Western Blot: PHYH Antibody (1F2-5B9) [H00005264-M01]

Western Blot: PHYH Antibody (1F2-5B9) [H00005264-M01] - Analysis of PHYH expression in transfected 293T cell line by PHYH monoclonal antibody (M01), clone 1F2-5B9.Lane 1: PHYH transfected lysate(38.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PHYH Antibody (1F2-5B9) [H00005264-M01] - Detection limit for recombinant GST tagged PHYH is 0.1 ng/ml as a capture antibody.

Applications for PHYH Antibody (1F2-5B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHYH

This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically, this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

LNAP1, PAHXLN1, peroxisomal, PhyH, PHYH1, phytanoyl-CoA 2-hydroxylase, phytanoyl-CoA hydroxylase, phytanoyl-CoA hydroxylase (Refsum disease), RD

Entrez Gene IDs

5264 (Human)

Gene Symbol

PHYH

OMIM

266500 (Human)

UniProt

AAH29512 (Human)

Additional PHYH Products

Product Documents for PHYH Antibody (1F2-5B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHYH Antibody (1F2-5B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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