PHYHD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81187
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:VLEGFLSAEECVAMQQRIGEIVAEMDVPLHCRTEFSTQEEEQLRAQGSTDYFLSSGDKIRFFFEKGVFDEKGNFLVPPEKSINKIGHALHAHDPVFKSITHSFKVQTLARSLGLQMPVVV
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHYHD1 Antibody
![Western Blot: PHYHD1 Antibody [NBP1-81187]](https://resources.bio-techne.com/images/products/PHYHD1-Antibody-Western-Blot-NBP1-81187-img0004.jpg "Western Blot: PHYHD1 Antibody [NBP1-81187]")
Western Blot: PHYHD1 Antibody [NBP1-81187]
Western Blot: PHYHD1 Antibody [NBP1-81187] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)
![Immunocytochemistry/ Immunofluorescence: PHYHD1 Antibody [NBP1-81187]](https://resources.bio-techne.com/images/products/PHYHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81187-img0005.jpg "Immunocytochemistry/ Immunofluorescence: PHYHD1 Antibody [NBP1-81187]")
Immunocytochemistry/ Immunofluorescence: PHYHD1 Antibody [NBP1-81187]
Immunocytochemistry/Immunofluorescence: PHYHD1 Antibody [NBP1-81187] - Staining of human cell line Hep G2 shows positivity in nucleus but excluded from the nucleoli. Antibody staining shown in green.Applications for PHYHD1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHYHD1
Alternate Names
MGC16638, phytanoyl-CoA dioxygenase domain containing 1, phytanoyl-CoA dioxygenase domain-containing protein 1
Gene Symbol
PHYHD1
Additional PHYHD1 Products
Product Documents for PHYHD1 Antibody
Product Specific Notices for PHYHD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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