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PI 3-Kinase C2 beta Antibody (1H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005287-M06

Novus Biologicals, part of Bio-Techne
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H00005287-M06-100ug

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PIK3C2B (NP_002637, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSTQDNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQG

Specificity

PIK3C2B - phosphoinositide-3-kinase, class 2, beta polypeptide (1H4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PI 3-Kinase C2 beta Antibody (1H4)

Western Blot: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06]

Western Blot: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06]

Western Blot: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06] - Analysis of PIK3C2B expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06]

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06]

Immunocytochemistry/Immunofluorescence: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06] - Analysis of monoclonal antibody to PIK3C2B on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: PI 3-Kinase C2 beta Antibody (1H4) [H00005287-M06] - Detection limit for recombinant GST tagged PIK3C2B is approximately 0.03ng/ml as a capture antibody.

Applications for PI 3-Kinase C2 beta Antibody (1H4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PI 3-Kinase C2 beta

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq]

Long Name

Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-containing Subunit beta

Alternate Names

C2-PI3K, Phosphoinositide 3-kinase-C2-beta, PI 3Kinase C2 beta, PIK3C2B, PTDINS-3-kinase C2 beta

Entrez Gene IDs

5287 (Human)

Gene Symbol

PIK3C2B

Additional PI 3-Kinase C2 beta Products

Product Documents for PI 3-Kinase C2 beta Antibody (1H4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PI 3-Kinase C2 beta Antibody (1H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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