PI 3-Kinase p85 beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56360

Novus Biologicals, part of Bio-Techne
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NBP2-56360
NBP2-56360-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PI 3-Kinase p85 beta Antibodies »

Product Summary for PI 3-Kinase p85 beta Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PI 3-Kinase p85 beta Antibody

Western Blot: PI 3-Kinase p85 beta Antibody [NBP2-56360]

Western Blot: PI 3-Kinase p85 beta Antibody [NBP2-56360]

Western Blot: PI 3-Kinase p85 beta Antibody [NBP2-56360] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 beta Antibody [NBP2-56360]

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 beta Antibody [NBP2-56360]

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 beta Antibody [NBP2-56360] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody [NBP2-56360]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody [NBP2-56360]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody [NBP2-56360] - Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Applications for PI 3-Kinase p85 beta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p85 beta

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Long Name

Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta

Alternate Names

p85-beta, PI 3Kinase p85 beta, PIK3R2

Gene Symbol

PIK3R2

Additional PI 3-Kinase p85 beta Products

Product Documents for PI 3-Kinase p85 beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PI 3-Kinase p85 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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