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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Phosphatidylinositol 4-Kinase, Catalytic, beta using the numbering given in entry CAA09495.1 (GeneID 5298).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PI4KB/PI4KIII beta Antibody

Immunoprecipitation: PI4KB/PI4KIII beta Antibody [NBP2-12814]

Immunoprecipitation: PI4KB/PI4KIII beta Antibody [NBP2-12814]

Immunoprecipitation: PI4KB/PI4KIII beta Antibody [NBP2-12814] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PI4KB antibody NBP2-12814 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PI4KB was also immunoprecipitated by rabbit anti-PI4KB antibody NBP2-12815, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Applications for PI4KB/PI4KIII beta Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PI4KB/PI4KIII beta

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking

Long Name

Phosphatidylinositol 4-kinase beta

Alternate Names

NPIK, PI4K-beta, PI4K92, PI4KB, PI4Kbeta, PI4KIII, PIK4CB

Gene Symbol

PI4KB

Additional PI4KB/PI4KIII beta Products

Product Documents for PI4KB/PI4KIII beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PI4KB/PI4KIII beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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