PIAS1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77158

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PIAS1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1. The immunogen is located within the last 50 amino acids of PIAS1. Amino Acid Squence: SNSLRESHSHTVTNR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIAS1 Antibody - BSA Free

Western Blot: PIAS1 AntibodyBSA Free [NBP1-77158]

Western Blot: PIAS1 AntibodyBSA Free [NBP1-77158]

Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: PIAS1 Antibody - BSA Free [NBP1-77158]

Immunohistochemistry: PIAS1 Antibody - BSA Free [NBP1-77158]

Immunohistochemistry: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue at 20 ug/mL.
Immunohistochemistry-Paraffin: PIAS1 Antibody - BSA Free [NBP1-77158]

Immunohistochemistry-Paraffin: PIAS1 Antibody - BSA Free [NBP1-77158]

Immunohistochemistry-Paraffin: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue with PIAS1 antibody at 5 ug/mL.
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Applications for PIAS1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP1-77158 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIAS1

The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.

Long Name

Protein Inhibitor of Activated STAT 1

Alternate Names

DDXBP1, GU/RH-II, ZMIZ3

Gene Symbol

PIAS1

UniProt

NP_057250 (Human)

Additional PIAS1 Products

Product Documents for PIAS1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIAS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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