PIEZO1 Antibody - BSA Free

Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78537]

Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.

Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] -

Immunohistochemistry: PIEZO1 Antibody [NBP1-78537] - Histological analysis and the expression of PIEZO1 (NBP1-78537) and collage II (NBP1-77795) in human nucleus pulposus tissues. A, B Hematoxylin and eosin (H&E) staining in human NP tissues of the control or the IVDD group (200×). C, D PIEZO1 immunostaining in human NP tissues of the control or the IVDD group (100×). E The percentage of PIEZO1 positive cells to all cells in the field (%). F, G Collagen II immunostaining in human NP tissues of the control or the IVDD group (100×). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35606793/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] -

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78537] - Green: 2 hours at RT with CLTC antibody diluted 1:100 in PBS-Triton 0.2%X-100/BSA 1%. Red: Phalloidin Blue: DAPI. Image from verified customer review.

Simple Western: PIEZO1 Antibody - BSA Free [NBP1-78537] -

Simple Western: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression profiling, cellular mechanotransduction, and biomechanical correlation analysis across brain regions of different donors. The relative abundance of (A) Piezo1, (B) YAP, (C) pYAP, and (D) beta-catenin in the regions of WM (magenta), GW junction (purple), and pons (green) for individual donors (D1-4). (E, top left) Spearman correlation between Piezo1 relative fluorescence units (RFU) and stiffness; GW Junction (orange) ns, WM (blue) r = -0.5341, p < 0.05, pons (green) ns. (E, top right) Correlation between Piezo1 regional RFU and spring term; GW junction r= 0.8791, and WM r = -0.5341, p < 0.05. (E, bottom left) Correlation between Piezo1 regional RFU and decay term (b); WM r = -0.6758, and pons r = 0.7692, p < 0.05. (E, bottom right) Correlation between Piezo1 regional RFU and equilibrium stress term; WM r = -0.8571, pons r = 0.7198, p < 0.05. (F) Comparison of protein expressed based on brain region for all donors, *p < 0.05 Image collected and cropped by CiteAb from the following publication (https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-023-01071-5 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PIEZO1 Antibody - BSA Free [NBP1-78537] -

Western Blot: PIEZO1 Antibody - BSA Free [NBP1-78537] - Expression & distribution of Piezo channels in mouse lenses. (A). RT-PCR-based confirmation of Piezo1 & Piezo2 expression in P1 & P30 mouse lenses. (B). qRT-PCR analysis revealed a relatively much higher level of Piezo1 expression in the lens (P30) compared to Piezo2. (C). Total lysates (800× g supernatants; 75 µg protein) derived from the P1, P14, & P16 mouse lenses analyzed using a Piezo1 polyclonal antibody exhibited immunopositive bands with an expected molecular mass of >250 kDa & >150 kDa. There was also a prominent immunopositive band at >75 kDa in the P1 & P14 lenses, the levels of which appeared to be decreased in the P16 lenses. (D). Piezo1 immunopositive bands of >250 & >75 kDa were present predominantly in the lens fiber samples (P21 & P27) compared to the lens epithelium (P21). (E,F). Immunofluorescence analysis of Piezo1 in the P1 mouse lens (the sagittal plane of the cryosection) revealed that the protein distributes predominantly to lens fibers relative to the epithelium (boxed area in panel (E) was magnified & shown in panel (F)). (G) Shows background immunofluorescence with secondary antibody alone. GAPDH: Loading control; Epi: Epithelium; Bars: Image magnification. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35563101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78537] -

Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78537] - Piezo1 expression & cell function of human nucleus pulposus cells under mechanical stress. A NP cells received various mechanical stress treatment. Cell viability was measured by MTT assay. B Gene expression of Piezo1 was measured by real-time PCR. C, D Western blot analysis of Piezo1’s protein level. Total beta-actin served as loading controls. E, F Immunofluorescence staining analysis of Piezo1 expression. Relative fluorescent levels of Piezo1 were measured by Image J software. G Concentrations of pro-inflammatory cytokines TNF-alpha, IL-1 beta, & IL-6 in supernatants were measured by ELISA. H Mitochondrial membrane potential was measured by JC-1 probe & flow cytometer. I OCR of cells were measured by Seahorse XFe96 Extracellular Flux Analyzer at basal conditions & with serial administration of oligomycin, FCCP & rotenone. J–L Gene & protein expressions of P53 & P16. Total beta-actin served as loading controls. *p < 0.05. All experiments were repeated at least three times Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35606793), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PIEZO1 Antibody - BSA Free [NBP1-78537] -

Western Blot: PIEZO1 Antibody - BSA Free [NBP1-78537] - The piezo1-tdT mouse confirms the expression & distribution of Piezo1 in lens fibers. (A). The Piezo1-tdT mouse model expressing a fusion protein of Piezo1 & tdTomato (Piezo1-tdT) was used to determine the distribution pattern of Piezo1 in the mouse lens. Similar to what was found in the wild-type lenses (Figure 3), Piezo1-tdT exhibiting the expected molecular mass of >250 kDa was detected only in P30 lens homogenates derived from the Piezo1-tdT mice, but not in the wild-type lens. The positive control (lung tissue lysate from the Piezo1-tdT mice) also showed a robust expression of Piezo1-tdT. Lanes 1 & 2 represent two different loads of the total protein (75 & 150 µg, respectively). (B) The Piezo1-tdT fusion protein was detected predominantly in fiber cell lysates compared to lens epithelial lysates. (C). Immunofluorescence analysis revealed the Piezo1-tdT fusion protein distributing to lens fibers with localization to both the short & long arms of the hexagonal lens fibers (Left & middle panels are with low & high magnification, respectively). The right panel shows a second antibody (Alexa Flour 488) background control fluorescence staining in the Piezo1-tdT mouse lens section. Bars: Image magnification. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35563101), licensed under a CC-BY license. Not internally tested by Novus Biologicals.