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PIG3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87275

Novus Biologicals, part of Bio-Techne
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NBP1-87275
NBP1-87275-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MLAVHFDKPGGPENLYVKEVAKPSPGEGEVLLKVAASALNRADLMQRQGQYDPPPGASNILGLEASGHVAELGPGCQGHWKIGDTAMALLPGGGQAQYVTVPEGLLMPIPE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIG3 Antibody

Western Blot: PIG3 Antibody [NBP1-87275]

Western Blot: PIG3 Antibody [NBP1-87275]

Western Blot: PIG3 Antibody [NBP1-87275] - Analysis using Anti-TP53I3 antibody NBP1-87275 (A) shows similar pattern to independent antibody NBP1-87274 (B).
Immunocytochemistry/ Immunofluorescence: PIG3 Antibody [NBP1-87275]

Immunocytochemistry/ Immunofluorescence: PIG3 Antibody [NBP1-87275]

Immunocytochemistry/Immunofluorescence: PIG3 Antibody [NBP1-87275] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PIG3 Antibody [NBP1-87275]

Immunohistochemistry-Paraffin: PIG3 Antibody [NBP1-87275]

Immunohistochemistry-Paraffin: PIG3 Antibody [NBP1-87275] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for PIG3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIG3

May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. Has low NADPH-dependent diamine reductase activity

Alternate Names

p53-induced gene 3 protein, PIG3quinone oxidoreductase homolog, quinone oxidoreductase PIG3, tumor protein p53 inducible protein 3, Tumor protein p53-inducible protein 3

Gene Symbol

TP53I3

Additional PIG3 Products

Product Documents for PIG3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIG3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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