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PIGB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85856

Novus Biologicals, part of Bio-Techne
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NBP1-85856
NBP1-85856-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QRGTLDVMSHIQKVCYNNPNKSSASIFIMMPCHSTPYYSHVHCPLPMRFLQCPPDLTGKSHYLDEAD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIGB Antibody

Western Blot: PIGB Antibody [NBP1-85856]

Western Blot: PIGB Antibody [NBP1-85856]

Western Blot: PIGB Antibody [NBP1-85856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PIGB Antibody [NBP1-85856]

Immunohistochemistry-Paraffin: PIGB Antibody [NBP1-85856]

Immunohistochemistry-Paraffin: PIGB Antibody [NBP1-85856] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Western Blot: PIGB Antibody [NBP1-85856]

Western Blot: PIGB Antibody [NBP1-85856]

Western Blot: PIGB Antibody [NBP1-85856] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Applications for PIGB Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIGB

PIGB, also referred to as GPI mannosyltransferase 3, is 554 amino acids long and is approximately 65 kDa. PIGB is a mannosyltransferase that is implicated in the biosynthesis of GPI anchors. Current research on PIGB is being performed relating to several diseases and disorders including malaria, sleeping sickness, Griscelli syndrome and encephalomalacia. PIGB has also been shown to have interactions with TMED10, CDC40, DNAJA1, FNBP1L and EEF1A1 in pathways such as GPI anchor biosynthesis and metabolic pathways.

Alternate Names

EC 2.4.1, EC 2.4.1.-, GPI mannosyltransferase 3, GPI mannosyltransferase III, GPI-MT-III, MGC21236, phosphatidylinositol glycan anchor biosynthesis, class B, phosphatidylinositol glycan, class B, Phosphatidylinositol-glycan biosynthesis class B protein, PIG-B

Gene Symbol

PIGB

Additional PIGB Products

Product Documents for PIGB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIGB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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