PIGM Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94070
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 35-96 of human PIGM (NP_660150.1). YGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIGM Antibody - Azide and BSA Free
Western Blot: PIGM AntibodyAzide and BSA Free [NBP2-94070]
Western Blot: PIGM Antibody [NBP2-94070] - Analysis of extracts of various cell lines, using PIGM at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 120s.Immunocytochemistry/ Immunofluorescence: PIGM Antibody - Azide and BSA Free [NBP2-94070]
Immunocytochemistry/Immunofluorescence: PIGM Antibody [NBP2-94070] - Analysis of L929 cells using PIGM at dilution of 1:100. Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: PIGM Antibody - Azide and BSA Free [NBP2-94070]
Immunohistochemistry-Paraffin: PIGM Antibody [NBP2-94070] - Mouse kidney using PIGM Rabbit pAb (NBP2-94070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for PIGM Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIGM
Alternate Names
EC 2.4.1, EC 2.4.1.-, GPI mannosyltransferase 1, GPI mannosyltransferase I, GPI-MT-IPIG-M mannosyltransferase, MGC29896, phosphatidylinositol glycan anchor biosynthesis, class M, phosphatidylinositol glycan, class M, Phosphatidylinositol-glycan biosynthesis class M protein, PIG-M
Gene Symbol
PIGM
Additional PIGM Products
Product Documents for PIGM Antibody - Azide and BSA Free
Product Specific Notices for PIGM Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov