PIM1 Antibody (4C8D0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15863

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15863-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4C8D0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PIM1 Antibodies »

Product Summary for PIM1 Antibody (4C8D0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PIM1 (P11309). MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIM1 Antibody (4C8D0)

Western Blot: PIM1 Antibody (4C8D0) [NBP3-15863]

Western Blot: PIM1 Antibody (4C8D0) [NBP3-15863]

Western Blot: PIM1 Antibody (4C8D0) [NBP3-15863] - Western blot analysis of extracts of 22Rv1 cells, using PIM1 antibody (NBP3-15863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: PIM1 Antibody (4C8D0) [NBP3-15863]

Immunohistochemistry-Paraffin: PIM1 Antibody (4C8D0) [NBP3-15863]

Immunohistochemistry-Paraffin: PIM1 Antibody (4C8D0) [NBP3-15863] - Immunohistochemistry of paraffin-embedded mouse brain using PIM1 antibody (NBP3-15863) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PIM1 Antibody (4C8D0) [NBP3-15863]

Immunohistochemistry-Paraffin: PIM1 Antibody (4C8D0) [NBP3-15863]

Immunohistochemistry-Paraffin: PIM1 Antibody (4C8D0) [NBP3-15863] - Immunohistochemistry of paraffin-embedded human colon using PIM1 antibody (NBP3-15863) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for PIM1 Antibody (4C8D0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIM1

The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM]

Long Name

Proviral Integration Site 1

Alternate Names

EC 2.7.11.1, Oncogene PIM1, PIM, pim-1 kinase 44 kDa isoform, pim-1 oncogene, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1

Gene Symbol

PIM1

Additional PIM1 Products

Product Documents for PIM1 Antibody (4C8D0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIM1 Antibody (4C8D0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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