PIM2 Antibody (3V1S5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16789

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16789-100ul
NBP3-16789-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3V1S5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PIM2 Antibodies »

Product Summary for PIM2 Antibody (3V1S5)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 10-120 of human PIM2 (NP_006866.2). PAPPGTPTPPPGGKDREAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSPLSDSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERPL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIM2 Antibody (3V1S5)

Western Blot: PIM2 Antibody (3V1S5) [NBP3-16789]

Western Blot: PIM2 Antibody (3V1S5) [NBP3-16789]

Western Blot: PIM2 Antibody (3V1S5) [NBP3-16789] - Western blot analysis of extracts of various cell lines, using PIM2 Rabbit mAb (NBP3-16789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: PIM2 Antibody (3V1S5) [NBP3-16789]

Immunoprecipitation: PIM2 Antibody (3V1S5) [NBP3-16789]

Immunoprecipitation: PIM2 Antibody (3V1S5) [NBP3-16789] - Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug PIM2 antibody (NBP3-16789). Western blot was performed from the immunoprecipitate using PIM2 antibody (NBP3-16789) at a dilition of 1:1000.

Applications for PIM2 Antibody (3V1S5)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIM2

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Long Name

Proviral Integration Site 2

Alternate Names

EC 2.7.11.1, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2

Gene Symbol

PIM2

Additional PIM2 Products

Product Documents for PIM2 Antibody (3V1S5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PIM2 Antibody (3V1S5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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