PIM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26115

Novus Biologicals, part of Bio-Techne
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NBP2-26115
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PIM2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KDREAFEAEYRLGP corresponding to internal region (near N-Terminus) according to NP_006866.2.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PIM2 Antibody

Western Blot: PIM2 Antibody [NBP2-26115]

Western Blot: PIM2 Antibody [NBP2-26115]

Western Blot: PIM2 Antibody [NBP2-26115] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with NBP2-26115 (0.1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26115 (0.1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26115]

Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26115]

Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26115] - 5ug/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PIM2 Antibody

Application
Recommended Usage

Immunohistochemistry

0.5-1ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1:100 - 1:2000
Application Notes
Western blot: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody.
IHC: Paraffin embedded Human Heart.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIM2

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Long Name

Proviral Integration Site 2

Alternate Names

EC 2.7.11.1, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2

Entrez Gene IDs

11040 (Human); 18715 (Mouse); 317366 (Rat)

Gene Symbol

PIM2

Additional PIM2 Products

Product Documents for PIM2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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