Pin1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05424

Novus Biologicals, part of Bio-Techne
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NBP1-05424-0.025ml
NBP1-05424
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chicken, Equine

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pin1 Antibodies »

Product Specifications

Immunogen

Recombinant full length Pin1 purified from E. coli.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pin1 Antibody

Western Blot: Pin1 Antibody [NBP1-05424]

Western Blot: Pin1 Antibody [NBP1-05424]

Western Blot: Pin1 Antibody [NBP1-05424] - Western blot of whole HeLa cell homogenate stained with NBP1-05424, at dilution of 1:10000. A prominent band running with an apparent SDS-PAGE molecular weight of approx. 21kDa corresponds to Pin1.
Immunocytochemistry/ Immunofluorescence: Pin1 Antibody [NBP1-05424]

Immunocytochemistry/ Immunofluorescence: Pin1 Antibody [NBP1-05424]

Immunocytochemistry/Immunofluorescence: Pin1 Antibody [NBP1-05424] - HeLa cells stained with NBP1-05424 antibody to Pin-1 (1:1000 dilution, green) and monoclonal to fibrillarin, NB300-269 (red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.

Applications for Pin1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500 - 1:1000

Western Blot

1:10000
Application Notes
This Pin1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~21 kDa. Use in IHC-P reported in scientific literature (PMID: 24891219).

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

PBS. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.

Preservative

0.07% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pin1

Pin1 (peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is a small enzyme which is unique amongst all peptidyl-prolyl isomerases (PPIases) as it interacts with phosphorylated serine/threonine residues followed by a proline (pS/T-P). Localized in nucleus and cytoplasm, Pin1 interacts with STIL, KIF20B, NEK6, PRKX, BTK, DAPK1, ATCAY and phosphorylated RAF1. It specifically isomerizes pS/T-P peptide bonds of its target proteins and considering the number of phosphoproteins with pS/T-P motifs in cells, it is not surprising that Pin1 has such profound influence on multiple biological processes. ATM/ATR facilitates its phosphorylation upon DNA damage and DAPK1 mediated phosphorylation at Ser-71 leads to inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation. Pin1 is regarded as regulatory timer of cell signaling processes and it is implicated in transcription and enzymatic activity, protein phosphorylation, stability, processing, localization and interaction, cellular growth/proliferation, apoptosis and immune responses. Moreover, Pin1's deregulation contributes to many human pathologies such as aging, cancer, neurological disorders including Alzheimer's disease, autoimmune and inflammatory diseases.

Long Name

Protein [Peptidyl-prolyl cis/trans Isomerase] NIMA-interacting 1

Alternate Names

dod

Gene Symbol

PIN1

Additional Pin1 Products

Product Documents for Pin1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pin1 Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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