PIP Antibody (PIP/1571)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53226

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PIP/1571

Concentration

0.2 mg/ml

View all PIP Antibodies »

Product Specifications

Immunogen

Recombinant human PIP protein fragment (around aa 41-146) (exact sequence is proprietary) (Uniprot: P12273)

Localization

Cytoplasmic (Golgi bodies) and Secreted

Specificity

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Marker

Breast Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54451)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PIP Antibody (PIP/1571)

Western Blot: PIP Antibody (PIP/1571) [NBP2-53226]

Western Blot: PIP Antibody (PIP/1571) [NBP2-53226]

Western Blot: PIP Antibody (PIP/1571) [NBP2-53226] - Western Blot Analysis (A) Human Pancreas (B) HepG2 Cell lysate Using GCDFP-15 Monoclonal Antibody (PIP/1571).
Immunohistochemistry-Paraffin: PIP Antibody (PIP/1571) [NBP2-53226]

Immunohistochemistry-Paraffin: PIP Antibody (PIP/1571) [NBP2-53226]

Immunohistochemistry-Paraffin: PIP Antibody (PIP/1571) [NBP2-53226] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GCDFP-15 Monoclonal Antibody (PIP/1571).
Protein Array: PIP Antibody (PIP/1571) [NBP2-53226]

Protein Array: PIP Antibody (PIP/1571) [NBP2-53226]

Protein Array: PIP Antibody (PIP/1571) [NBP2-53226] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PIP Antibody (PIP/1571). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for PIP Antibody (PIP/1571)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PIP

The androgen and prolactin responsive prolactin-inducible protein (PIP), also known as Gross cystic disease fluid protein (GCDFP-15) is expressed in benign and malignant human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive PIP gene is expressed in benign and malignant breast tumor tissues and in such normal exocrine organs as sweat, salivary, and lacrimal glands. The cells within the body that produce PIP/GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found PIP to be a highly specific and sensitive marker for breast cancer.

Alternate Names

GCDFP15prolactin-inducible protein, GCDFP-15SABP, GPIP4gp17, Gross cystic disease fluid protein 15, prolactin-induced proteinSecretory actin-binding protein

Gene Symbol

PIP

UniProt

P12273 (Human)

Additional PIP Products

Product Documents for PIP Antibody (PIP/1571)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIP Antibody (PIP/1571)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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