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PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25603

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25603

Key Product Details

Species Reactivity

Human, Rat, Canine, Feline

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2011

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PIP5K1 alpha. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

Western Blot: PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free [NBP3-25603] -

Western Blot: PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free [NBP3-25603] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (NBP3-25603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

Western Blot: PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free [NBP3-25603] -

Western Blot: PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free [NBP3-25603] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (NBP3-25603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free [NBP3-25603] -

Immunocytochemistry/Immunofluorescence: PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free [NBP3-25603] - PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PIP5K1A stained by PIP5K1A antibody [HL2011] (NBP3-25603) diluted at 1:500. Blue: Fluoroshield with DAPI .

Applications for PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIP5K1 alpha

Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2

Alternate Names

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, EC 2.7.1, EC 2.7.1.68, Phosphatidylinositol-4-phosphate 5-kinase type I alpha, phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, PIP5K1-alpha, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha

Gene Symbol

PIP5K1A

Additional PIP5K1 alpha Products

Product Documents for PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIP5K1 alpha Antibody (HL2011) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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