PIP5K1C Antibody (PSH01-43)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32824
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-43
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human PIP5K1C aa 31-470 / 668. (Uniprot: O60331)
Localization
Cell membrane, Endomembrane system, Cytoplasm, Cell junction, focal adhesion, adherens junction, Cell projection, ruffle membrane, phagocytic cup, uropodium; Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PIP5K1C Antibody (PSH01-43)
Western Blot: PIP5K1C Antibody (PSH01-43) [NBP3-32824]
Western Blot: PIP5K1C Antibody (PSH01-43) [NBP3-32824] - Western blot analysis of PIP5K1C on different lysates with Rabbit anti-PIP5K1C antibody (NBP3-32824) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: A431 cell lysate
Lane 5: SiHa cell lysate
Lane 6: A549 cell lysate
Lane 7: LoVo cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 73 kDa
Observed band size: 87/90 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32824) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for PIP5K1C Antibody (PSH01-43)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIP5K1C
Long Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
Alternate Names
EC 2.7.1.68, KIAA0589, PIP5K1gamma
Gene Symbol
PIP5K1C
Additional PIP5K1C Products
Product Documents for PIP5K1C Antibody (PSH01-43)
Product Specific Notices for PIP5K1C Antibody (PSH01-43)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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