PIP5K2 alpha Antibody (OTI3D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03492

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D3

Concentration

0.9 mg/ml

View all PIP5K2 alpha Antibodies »

Product Summary for PIP5K2 alpha Antibody (OTI3D3)

Immunogen

Full length human recombinant protein of human PIP4K2A (NP_005019) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIP5K2 alpha Antibody (OTI3D3)

Western Blot: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492]

Western Blot: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492]

Western Blot: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIP5K2 alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIP5K2 alpha.
Immunohistochemistry-Paraffin: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492]

Immunohistochemistry-Paraffin: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492]

Immunohistochemistry-Paraffin: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492] - Staining of paraffin-embedded Human lymphoma tissue using anti-PIP5K2 alpha mouse monoclonal antibody.
Flow Cytometry: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492]

Flow Cytometry: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492]

Flow Cytometry: PIP5K2 alpha Antibody (OTI3D3) [NBP2-03492] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIP5K2 alpha antibody, and then analyzed by flow cytometry.
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Applications for PIP5K2 alpha Antibody (OTI3D3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIP5K2 alpha

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq].

Alternate Names

1-phosphatidylinositol-4-phosphate kinase, 1-phosphatidylinositol-4-phosphate-5-kinase, 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha, Diphosphoinositide kinase 2-alpha, EC 2.7.1, EC 2.7.1.149, FLJ13267, phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, Phosphatidylinositol-5-phosphate 4-kinase type II alpha, phosphatidylinositol-5-phosphate 4-kinase type-2 alpha, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha, PI(5)P 4-kinase type II alpha, PIP4KII-alpha, PIP5K2, PIP5K2API5P4KA, PIP5KIIA, PIP5KIIalpha, PIP5KII-alpha, PIP5KIII, PIPK, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PtdIns(5)P-4-kinase isoform 2-alpha, type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform

Entrez Gene IDs

5305 (Human)

Gene Symbol

PIP4K2A

Additional PIP5K2 alpha Products

Product Documents for PIP5K2 alpha Antibody (OTI3D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIP5K2 alpha Antibody (OTI3D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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