PIP5K2B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44261
Key Product Details
Species Reactivity
Human, Mouse, Rat, Chicken, Primate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PIP5K2B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B. (Uniprot: Q8TCG2)
Reactivity Notes
Cross-reactivity with PIP5K2B from other sources has not been determined
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for PIP5K2B Antibody
![Western Blot: PIP5K2B Antibody [NBP1-44261]](https://resources.bio-techne.com/images/products/PIP5K2B-Antibody-Western-Blot-NBP1-44261-img0003.jpg "Western Blot: PIP5K2B Antibody [NBP1-44261]")
Western Blot: PIP5K2B Antibody [NBP1-44261]
Western Blot: PIP5K2B Antibody [NBP1-44261] - Lane 1: Mouse Brain homogenate samples. Load: 10 ug per lane. Primary antibody: PIP5K2B antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.Applications for PIP5K2B Antibody
Application
Recommended Usage
ELISA
1:20000-1:60000
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:100-1:500
Western Blot
0.2 ug/ml
Application Notes
This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~54 kDa corresponding to the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: PIP5K2B
Alternate Names
1-phosphatidylinositol-4-phosphate kinase, 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, EC 2.7.1, EC 2.7.1.149, phosphatidylinositol-4-phosphate 5-kinase, type II, beta, Phosphatidylinositol-5-phosphate 4-kinase type II beta, phosphatidylinositol-5-phosphate 4-kinase type-2 beta, phosphatidylinositol-5-phosphate 4-kinase, type II, beta, PI(5)P 4-kinase type II beta, PI5P4KB, PIP4KII-beta, PIP5K2B, PIP5KIIB, PIP5KIIbeta, PTDINS(4)P-5-kinase, PtdIns(5)P-4-kinase isoform 2-beta
Entrez Gene IDs
8396 (Human)
Gene Symbol
PIP4K2B
UniProt
Additional PIP5K2B Products
Product Documents for PIP5K2B Antibody
Product Specific Notices for PIP5K2B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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