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PIP5K2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44261

Novus Biologicals, part of Bio-Techne
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NBP1-44261

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Primate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PIP5K2B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B. (Uniprot: Q8TCG2)

Reactivity Notes

Cross-reactivity with PIP5K2B from other sources has not been determined

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PIP5K2B Antibody

Western Blot: PIP5K2B Antibody [NBP1-44261]

Western Blot: PIP5K2B Antibody [NBP1-44261]

Western Blot: PIP5K2B Antibody [NBP1-44261] - Lane 1: Mouse Brain homogenate samples. Load: 10 ug per lane. Primary antibody: PIP5K2B antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.

Applications for PIP5K2B Antibody

Application
Recommended Usage

ELISA

1:20000-1:60000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Western Blot

0.2 ug/ml
Application Notes
This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~54 kDa corresponding to the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PIP5K2B

PIP5K2B - phosphatidylinositol-4-phosphate 5-kinase, type II, beta.Functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization.

Alternate Names

1-phosphatidylinositol-4-phosphate kinase, 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, EC 2.7.1, EC 2.7.1.149, phosphatidylinositol-4-phosphate 5-kinase, type II, beta, Phosphatidylinositol-5-phosphate 4-kinase type II beta, phosphatidylinositol-5-phosphate 4-kinase type-2 beta, phosphatidylinositol-5-phosphate 4-kinase, type II, beta, PI(5)P 4-kinase type II beta, PI5P4KB, PIP4KII-beta, PIP5K2B, PIP5KIIB, PIP5KIIbeta, PTDINS(4)P-5-kinase, PtdIns(5)P-4-kinase isoform 2-beta

Entrez Gene IDs

8396 (Human)

Gene Symbol

PIP4K2B

UniProt

Additional PIP5K2B Products

Product Documents for PIP5K2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIP5K2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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