Pirin Antibody (4D1)
Novus Biologicals, part of Bio-Techne | Catalog # H00008544-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4D1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PIR (AAH02517, 1 a.a. ~ 290 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTRKSKIGN
Specificity
PIR (4D1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Pirin Antibody (4D1)
![Western Blot: Pirin Antibody (4D1) [H00008544-M03]](https://resources.bio-techne.com/images/products/Pirin-Antibody-4D1-Western-Blot-H00008544-M03-img0003.jpg "Western Blot: Pirin Antibody (4D1) [H00008544-M03]")
Western Blot: Pirin Antibody (4D1) [H00008544-M03]
Western Blot: Pirin Antibody (4D1) [H00008544-M03] - Analysis of PIR expression in transfected 293T cell line by PIR monoclonal antibody (M03), clone 4D1. Lane 1: PIR transfected lysatE (32.113 KDa). Lane 2: Non-transfected lysate.![Western Blot: Pirin Antibody (4D1) [H00008544-M03]](https://resources.bio-techne.com/images/products/Pirin-Antibody-4D1-Western-Blot-H00008544-M03-img0002.jpg "Western Blot: Pirin Antibody (4D1) [H00008544-M03]")
Western Blot: Pirin Antibody (4D1) [H00008544-M03]
Western Blot: Pirin Antibody (4D1) [H00008544-M03] - Western blot analysis of PIR over-expressed 293 cell line, cotransfected with PIR Validated Chimera RNAi or non-transfected control. Blot probed with H00008544-M03. GAPDH (36.1 kDa) used as loading control.Applications for Pirin Antibody (4D1)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Pirin
Alternate Names
EC 1.13.11.24, pirin, pirin (iron-binding nuclear protein), Probable quercetin 2,3-dioxygenase PIR, Probable quercetinase
Entrez Gene IDs
8544 (Human)
Gene Symbol
PIR
OMIM
603329 (Human)
UniProt
Additional Pirin Products
Product Documents for Pirin Antibody (4D1)
Product Specific Notices for Pirin Antibody (4D1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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