Pirin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25053

Novus Biologicals, part of Bio-Techne
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NBP3-25053
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pirin Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Pirin using the following amino acid sequence: SFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFERAKTWKSK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pirin Antibody

Pirin Antibody Immunocytochemistry/Immunofluorescence: Pirin Antibody [NBP3-25053]

Immunocytochemistry/Immunofluorescence: Pirin Antibody [NBP3-25053]

Staining of human cell line U2OS shows localization to nucleoplasm & cytosol.
Pirin Antibody Western Blot: Pirin Antibody [NBP3-25053]

Western Blot: Pirin Antibody [NBP3-25053]

Analysis in human cell line RT-4 and human cell line U-251 MG.

Applications for Pirin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pirin

Pirin encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Alternate Names

EC 1.13.11.24, pirin, pirin (iron-binding nuclear protein), Probable quercetin 2,3-dioxygenase PIR, Probable quercetinase

Gene Symbol

PIR

Additional Pirin Products

Product Documents for Pirin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pirin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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